Journal: Bioengineering

Article Title: Multiphotonic Ablation and Electro-Capacitive Effects Exhibited by Candida albicans Biofilms

doi: 10.3390/bioengineering11040333

Figure Lengend Snippet: ( a ) Brightfield microscopy of C. albicans in vitro biofilm; ( b ) fungal cell wall α-D-mannosyl residues labeled with Concanavalin A stain, observed by epifluorescence microscopy; ( c ) biofilm architecture observed by scanning electron microscopy. In all cases, in vitro biofilm was started with an inoculum of 1 × 10 6 blastoconidia/mL and was incubated at 37 °C for 24 h.

Article Snippet: For brightfield microscopy, a vitro biofilm was placed over sterile glass coverslips in the bottom of a 12-well polystyrene plate and incubated at 37 °C for 24 h. After incubation, culture media were discarded, and the studied biofilm was washed twice with PBS 1X, coverslip, recovered, and observed in brightfield microscopy (Primo Star, Carl Zeiss, Jena, Germany).

Techniques: Microscopy, In Vitro, Labeling, Staining, Epifluorescence Microscopy, Electron Microscopy, Incubation